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Literature summary extracted from

  • Jiang, R.; Krzycki, J.A.
    PylSn and the homologous N-terminal domain of pyrrolysyl-tRNA synthetase bind the tRNA that is essential for the genetic encoding of pyrrolysine (2012), J. Biol. Chem., 287, 32738-32746.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.26 expressed in Escherichia coli BL21(DE3) cells Methanosarcina barkeri
6.1.1.26 expressed in Escherichia coli BL21(DE3) cells Desulfitobacterium hafniense

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.26 additional information truncation of the N-terminal region of PylS (DELTA92PylS) eliminates detectable tRNAPyl binding, but not catalytic activity Methanosarcina barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.26 0.044
-
 L-pyrrolysine wild type enzyme, at 37°C in 50 mM KCl, 10 mM MgCl2, 5 mM ATP, and 5 mM dithiothreitol in 10 mM HEPES buffer, pH 7.2 Methanosarcina barkeri
6.1.1.26 0.044
-
 L-pyrrolysine wild type enzyme, at 37°C in 50 mM KCl, 10 mM MgCl2, 5 mM ATP, and 5 mM dithiothreitol in 10 mM HEPES buffer, pH 7.2 Desulfitobacterium hafniense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl Methanosarcina barkeri
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl Desulfitobacterium hafniense
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.26 Desulfitobacterium hafniense
-
-
-
6.1.1.26 Methanosarcina barkeri
-
-
-
6.1.1.26 Methanosarcina barkeri Q6WRH6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.26 nickel-activated HiTrap column chromatography Methanosarcina barkeri
6.1.1.26 nickel-activated HiTrap column chromatography Desulfitobacterium hafniense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl
-
 Methanosarcina barkeri AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl
-
 Desulfitobacterium hafniense AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl the N-terminal region of PylS influences complex formation with tRNAPyl Methanosarcina barkeri AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.26 PylRS
-
 Methanosarcina barkeri
6.1.1.26 PylRS
-
 Desulfitobacterium hafniense
6.1.1.26 PylS
-
 Methanosarcina barkeri
6.1.1.26 PylS
-
 Desulfitobacterium hafniense
6.1.1.26 PylSn
-
 Methanosarcina barkeri
6.1.1.26 pyrrolysyl-tRNA synthetase
-
 Methanosarcina barkeri
6.1.1.26 pyrrolysyl-tRNA synthetase
-
 Desulfitobacterium hafniense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.26 0.1
-
 L-pyrrolysine wild type enzyme, at 37°C in 50 mM KCl, 10 mM MgCl2, 5 mM ATP, and 5 mM dithiothreitol in 10 mM HEPES buffer, pH 7.2 Methanosarcina barkeri
6.1.1.26 0.325
-
 L-pyrrolysine wild type enzyme, at 37°C in 50 mM KCl, 10 mM MgCl2, 5 mM ATP, and 5 mM dithiothreitol in 10 mM HEPES buffer, pH 7.2 Methanosarcina barkeri
6.1.1.26 0.325
-
 L-pyrrolysine wild type enzyme, at 37°C in 50 mM KCl, 10 mM MgCl2, 5 mM ATP, and 5 mM dithiothreitol in 10 mM HEPES buffer, pH 7.2 Desulfitobacterium hafniense

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.26 ATP
-
 Methanosarcina barkeri
6.1.1.26 ATP
-
 Desulfitobacterium hafniense